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yitA [2019-07-31 08:28:05]
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yitA [2019-07-31 08:28:05]

sulfate adenylyltransferase
locus
BSU_10920
pI
7.55
mw
43.09 kDa
protein length
389 aa Sequence Blast
gene length
1170 bp Sequence Blast
function
sulfate reduction and activation
product
sulfate adenylyltransferase
essential
no
ec
2.7.7.4
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    1,170,473 → 1,171,642

    The protein

    Catalyzed reaction/ biological activity

  • ATP + sulfate = diphosphate + adenylyl sulfate (according to Swiss-Prot)

    • Protein family

  • sulfate adenylyltransferase family (with [protein|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|Sat], according to UniProt)

    • Paralogous protein(s)

  • [protein|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|Sat]

    • Structure

  • [PDB|1R6X] (from yeast, 41% identity) [pubmed|14983089]

    • Expression and Regulation

    Operons

    genes
    [gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]
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    Biological materials

    Mutant

  • MGNA-A500 (yitA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/500 NBRP B. subtilis, Japan]
  • BKE10920 (Δ[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGTTCAACCTCCATT, downstream forward: _UP4_AAGCAAAACAGCGGGGAATT
  • BKK10920 (Δ[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGTTCAACCTCCATT, downstream forward: _UP4_AAGCAAAACAGCGGGGAATT

    • References

  • 15699190,15383836,14983089